منابع مشابه
The biosynthesis of 6-methylsalicylic acid. Crude enzyme systems from early and late producing strains of Penicillium patulum.
Two strains of Penicillium pafulum have been isolated from cultures of NRRL 21598. One produces 6-methylsalicyclic acid (MSA) very soon after transfer of mycelium from a germinating medium to Czapek-Dox medium and is designated an early MSA strain. In the other, 6-methylsalicyclic acid production begins only after 24 to 36 hours on Czapek-Dox medium, and it is designated a late MSA strain. Cell...
متن کاملCloning and characterization of novel methylsalicylic acid synthase gene involved in the biosynthesis of isoasperlactone and asperlactone in Aspergillus westerdijkiae.
Aspergillus westerdijkiae is the main producer of several biologically active polyketide metabolites including isoasperlactone and asperlactone. A 5298bp polyketide synthase gene "aomsas" has been cloned in Aspergillus westerdijkiae by using gene walking approach and RACE-PCR. The predicted amino acid sequence of aomsas shows an identity of 40-56% with different methylsalicylic acid synthase ge...
متن کاملHidden function of catalytic domain in 6-methylsalicylic acid synthase for product release.
Functional investigation of the proposed dehydratase domain of ATX, a 6-methylsalicylic acid synthase from Aspergillus terreus, revealed that the domain is not involved in dehydration of the beta-hydroxytriketide intermediate tethered on the acyl carrier protein but catalyzes thioester hydrolysis to release the product from the acyl carrier protein. Thus, we renamed this domain the thioester hy...
متن کاملPurification and properties of 6-methylsalicylic acid synthase from Penicillium patulum.
6-Methylsalicylic acid synthase has been isolated in homogeneous form from Penicillium patulum grown in liquid culture from a spore inoculum. The enzyme is highly susceptible to proteolytic degradation in vivo and in vitro, but may be stabilized during purification by incorporating proteinase inhibitors in the buffers. The enzyme exists as a homotetramer of M(r) 750,000, with a subunit M(r) of ...
متن کاملInsights into 6‐Methylsalicylic Acid Bio‐assembly by Using Chemical Probes
Chemical probes capable of reacting with KS (ketosynthase)-bound biosynthetic intermediates were utilized for the investigation of the model type I iterative polyketide synthase 6-methylsalicylic acid synthase (6-MSAS) in vivo and in vitro. From the fermentation of fungal and bacterial 6-MSAS hosts in the presence of chain termination probes, a full range of biosynthetic intermediates was isola...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)96083-7